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Hello, we have an AKTA Avant that we keep at room temperature. We would like to be able to use it for preps at 4 degrees, so we would like to keep the FPLC columns (type 10/30 from GE) in the cold. We were thinking of buying a circulating water bath and connect it somehow to the columns through a jacket. Has anybody had any experience with that? We looked also into buying the hot pocket from Fisher Scientific but it is a bit out of our budget. Any suggestions are very welcome. Chiara POSTDOC Structural Biology of Bacterial secretion Department of Structural Biology and Chemistry INSTITUT PASTEUR 25 rue du Docteur Roux 75015 Paris FRANCE Dr. Chiara Rapisarda Tel : +33 (0)1.40.61.35.31 Fax :+33 (0)1.45.68.88.36 Email: [log in to unmask]