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Dear Roberto, I think you should try different special orientations of your crystals in the loop to find the one in which the measurable reflections stay away from the slow moving region (usually aligned with the beamstop holder, so horizontal in your image), in which reflections cannot be acccurately measured and are discarded by integration programs. This way you wont at least lose more of your precious reflections. Generally Id recommend not to discard any measurable reflection irrespective of very bad overall statistics. Best, Matthias ----------------------------------------- Dr. Matthias Zebisch Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK Phone (+44) 1865 287549; Fax (+44) 1865 287547 Email [log in to unmask] Website http://www.strubi.ox.ac.uk ----------------------------------------- On 2/25/2015 10:07 PM, Orru, Roberto wrote: > Dear All, > > My crystals are heavily affected by diffraction anisotropy as you can see in the images attached. I have been using the server at UCLA (http://services.mbi.ucla.edu/anisoscale/) to treat the datasets, but I was wondering if there is any suggestion on how to setup the strategy for the data collections and for the following integration. > > Thanks in advance, > Roberto