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Dear SPM experts I have been working on SPM to analyze SPECT images and find abnormalities by comparing SPECT images of the patients with the normal control group images. I am completely new in SPM. For preprocessing I perform Coregistration, Normalization and Smoothing. After that for model specification I don't know which design (sample t-test, ANOVA. ) to choose? In two sample t-test, for example, there exists two mandatory selections “Group 1 scans”and“Group 2 scans”. Now my question is this: What (patient or control group) should I select for each above-mentioned group? Then for the results section I should define contrast (t contrast or f contrast). Again, I don't know how to define it? I would greatly appreciate if kindly help me in this case. Best. -- Negar Chabi Msc of medical engineering Persian Gulf Nuclear Medicine Research Center Bushehr University of Medical sciences