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Hello all, I have finished TBSS pipeline according to user guide ,and got some significant regions .However , I still have the following questions to ask for help . Before that I am sorry about my poor English . 1. Which atlases of FSL correspond to JHU-ICBM-DTI-81 White Matter and Juelich Histological atlases ,which were used to label clusters with significant FA alterations . 2. How to extract the FA value of every subject and average FA value of per group , and then plot something similar to figure 1? 3. How significant difference regions can be made into ROIs , and these ROI can be applied to every subjects ? or say how to implement a ROI-based TBSS? 4. Could TBSS make analysis with some covariants(e.g. age,weight)? If so ,please tell me way because I just make simple analysis whitout any covariant following user guide . 5. Could TBSS conduct correlation analysis between FA and covariants(e.g. age,weight) ,and get correlation coefficient r and P ,as shown in figure 3 ? Best regards. Xin-Fa Shi.