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First I want to say that I am finding the Follow Shift Changes tool really helpful. However, I was wondering what the procedure is for determining an overall Kd for a particular titration? At the moment, I can select a number of peaks I wish to follow and use for the calculation, and it gives me a Kd and error for each of those residues, but how should one use this information to find the overall Kd for the interaction? Currently, I am exporting the raw shifts, doing the shift sum calculations in Excel and fitting the results in Prism to find a global shared Kd value, and the standard error, but obviously it would be easier if it could be done in Analysis. Or am I missing something in how Kds should be calculated from NMR titrations? thanks, Kate