Hi Ayan,

Try 3V: 
http://3vee.molmovdb.org

It has worked nicely for me. You'll need to delete the substrate/inhibitor from your coordinate file for one of the runs. 

Cheers,
Chris

On Saturday, January 10, 2015, Bio Physics <[log in to unmask]> wrote:

Hello CCP4bb:

Help me to advice any program/server to calculate active site volume without substrate/inhibitor and volume of the substrate/inhibitor itself. 

Q-site-finder website is not working.

Thank you,

Ayan