Dear all,
I'm doing Hg SAD phasing for the first time, and I met some problem:
1. How to assess the anomalous signal after I process the data and get my mtz file?
My labmate doesn't scan the fluorescence signal. Actually, I don't quite understand why we use fluorescence to detect anomalous signal.
2. How to estimate the number of heave atom in one unit cell? by soaking heavy atom derivatives.
Best!
Lu Zuokun
Nankai University