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Dear Gongpu, Please follow the recommended procedures on the wiki and postprocess your half-maps. HTH, S > hi Sjors, > In the 3D auto-refine, I found out the final map run1_class001.mrc looks > filtered, but the two half map looks ok. I plot the power spectrum of the > final map on a log scale, and found out there is sharp drop. If I add two > half maps together and plot its power spectrum on a log scale, I don’t > see such sharp drop. I am wondering if you have any insight about what may > cause this. Can I just sum up the two half maps and use it as the final > map? Thank you very much. > > Best, > gongpu > -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres