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Hi Grigory, The answer is also simple: yes it does renumber like you describe. HTH, S > Dear Sjors, > > after autopicking I've compared the star file with references and output > particles star file with added Z-score. I found that _rlnClassNumber has > different values: e.g., 1,2,3,7,8,9,10 (total 7) in reference file and 1 > to > 7 in output file. > > My question is very simple: does Relion simply renumber all the > references? > Like 1=1, 2=2, 3=3, 4=7, 5=8, 6=9, 7=10? > > I'm asking this because I've used another reference-based piking program > and would like to run Z-score estimation in Relion. > > > Best regards, > Grigory Sharov > > Institute of Genetics and Molecular and Cellular Biology > Department of Structural Biology and Genomics > 1, rue Laurent Fries > 67404 Illkirch, France > tel. 03 69 48 51 00 > e-mail: [log in to unmask] > -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres