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Hi, yes this would be the minimal requirement. Cheers, Andreas Von: KUN HSIEN CHOU <[log in to unmask]> Antworten an: FSL - FMRIB's Software Library <[log in to unmask]> Datum: Samstag, 6. Dezember 2014 15:12 An: <[log in to unmask]> Betreff: Re: [FSL] TOPUP and eddy_correct Dear Andreas If I use 60 directions which generated from GPS command line tool , how could I use this kind of dataset with Eddy and topup ? When I read the website of Eddy tool , it seems like I also need b0 images which were acquired with reverse phase encoding. If I understand it correctly, I need to acquire additional B0 images with reverse phase encoding, is it right? Best Paul 2014-12-06 21:41 GMT+08:00 Andreas Bartsch <[log in to unmask]>: > Hi Paul, > >> >could I use the gps command line tool > > yes, you can. > >> >So do I need to acquire the full blip-up blip-down DTI dataset with 60 >> >directions > > You don't have to but you can to improve the SNR. > > Cheers, > Andreas > > Am 06.12.14 04:01 schrieb "Paul Chou" unter <[log in to unmask]>: > >> >Dear Jesper >> > >> >I have some questions about how to setup the diffusion protocol which can >> >be analyzed with eddy tool. I wish to acquire DTI dataset more than 30 >> >directions (I think the 60 directions which samples the diffusion sphere >> >uniformly is the good consideration , because i wish to perform some >> >crossing fiber modeling). After reading the website of eddy and topup >> >tools, I realize I also need to acquire the DTI dataset with reverse >> >phase encoding to perform DTI analysis with these great tools. So do I >> >need to acquire the full blip-up blip-down DTI dataset with 60 directions >> >(which samples the diffusion sphere uniformly) or I only need to acquire >> >the dataset with 60 directions only? >> > >> >By the way, could I use the gps command line tool to generate the 60 >> >directions which samples the diffusion sphere uniformly in my future >> >study ? >> > >> >Best >> > >> >Paul