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Hi Reza, Check the following reference: Cryoprotection properties of salts of organic acids: a case study for a tetragonal crystal of HEW lysozyme. Bujacz G, Wrzesniewska B, Bujacz A. Acta Crystallogr D Biol Crystallogr. 2010 Jul;66(Pt 7):789-96. Cheers, Karolina W dniu 2014-12-01 10:59, Reza Khayat napisał(a): > Hi, > > Has anyone used citrate as the sole cryoprotectant? If so, > what concentration was needed? > > Best wishes, > Reza > > Reza Khayat, PhD > Assistant Professor > The City College of New York > Department of Chemistry, MR-1135 > 160 Convent Avenue > New York, NY 10031 > Tel. (212) 650-6070 > www.khayatlab.org [1] Links: ------ [1] http://www.khayatlab.org