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Mohamed, You always list the sequence of what's actually in the crystal, e.g. 1-105. (Not: what's in the model or what the sequence of the full length protein is). Make sure that if there's any lingering residues from any affinity/purification tags they get included in the sequence too. Phil Jeffrey Princeton ________________________________________ From: CCP4 bulletin board [[log in to unmask]] on behalf of Mohamed Noor [[log in to unmask]] Sent: Tuesday, December 30, 2014 3:26 PM To: [log in to unmask] Subject: [ccp4bb] PDB deposition - sequence file Dear all The protein that was crystallized is only the first 105 residues of a 230-residue protein. In the structure, I can see density for residues 6-72. For deposition, should the whole native/biological sequence be deposited? Thanks. Mohamed