Hi Shengwei,Possibly, you can try DPABI (http://dpabi.org) VBM analysis, it has a step called "Reorient T1 Interactively".Also, the following New Segment + DARTEL happens automatically.Best,Chao-GanOn Wed, Nov 5, 2014 at 10:59 AM, Shengwei Zhang <[log in to unmask]> wrote:Any help is appreciated.I'm thinking of using spm_coreg first to do the rigid registration, but how can I extract the rigid transformation from this step? Do I need to invert this rigid transformation first before combining it to the DARTEL transformation?Does anyone have experience on how to implement this, in command-line or GUI mode?To avoid interpolation, I'm trying to first rigidly register all subjects to MNI space, run DARTEL on the aligned images, and then combine the rigid and DARTEL transformations and apply them to the raw space images.I'm using DARTEL in SPM8 for VBM study, and it is suggested that the input images should be roughly aligned to MNI space first before DARTEL processing.Hi SPM experts,Shengwei