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Dear SPM users, I have a doubt about the specification of my factorial design in a VBM analysis. I have a population divided into Patients and Healthy Controls (P and HC). I'd like to test whether there exist any differences between the two groups, removing the effect of gender, age, and TIV. Therefore I would do an ANCOVA, but the *Design *menu in the *Factorial design specification * only provides me with t-test, multiple regression, ANOVA, Full factorial, and Flexible factorial. So: 1. can I choose the One-way ANOVA, setting to YES the *Design -> One-way ANOVA -> ANCOVA *though they say that "This is only used for PET data"? 2. should I choose a *Two-sample t-test*, specifying three additional covariates? 3. since both the *t-test* and the *One-way ANOVA* allow inserting covariates, which are the difference between the two models? Thank you, elisa -- Elisa Veronese - PhD *Research Unit on Brain Imaging and Neuropsychology (RUBIN)* *Inter-University Center for Behavioral Neurosciences (ICBN)University of Udine and University of Verona, ItalyICBN Website: http://icbn.uniud.it/tiki-index.php <http://icbn.uniud.it/tiki-index.php>*