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Dear Wei, Thank you for reporting this bug (indeed). I have made a note on how to fix it here: http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Known_issue#Movie-extraction_with_first_frame_not_being_number_1 It will also be fixed in the next (1.4) release, but that will take at least another 6 months to be released. Cheers, Sjors On 10/30/2014 05:21 PM, Wei Lü wrote: > Hi Sjors, > > I found some strange behavior of Relion when I extract movie particles. > > In my raw data each movie contains 25 frames (1-25). > - If I let Relion to extract particles starting from the first frame, e.g. > 1-25 or 1-23, everything is fine. > - If I let Relion to exclude the first few frames, e.g. 3-23 or 4-23, what > I got is actually 3-21 or 4-20. Because the total number of frames are 21 > or 20, Relion just fills the rest with frame 21 (x2) or frame 20 (x3). > - Generally, regardless where are the first frame (m) and last frame (n), > it always extract frames from (m) to (n-m+1) and fill the rest with frame > (n-m+1). > > Do you think it is a bug? And could it be the reason that my refinement > is always at "Expanding..." ? > > Thanks, > Wei > -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres