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Hi Scott, Both scenarios can be dealt with in RELION, although the multi-reference 3D refinement will typically be more successful in scenario i) than in scenario 2). HTH, S > To follow on, it would appear two distinct scenarios can be discussed > here: i) a complex with/without ligand, i.e., multiple DEFINED > conformations; and ii) an inherently flexible molecule with a CONTINUUM of > different conformations (which is what I'm dealing with in EMAN). > > I assume RELION can handle both scenarios. Delving into the RELION > tutorial is high on my to-do list - is there an opportunity to explore the > conformational heterogeneity issue in the tutorial? > > Regards, > Scott > > > > Hi, > A similar question has been asked a few days ago. > I copy the thread below but you can also search archives on the ccpem > mailing list website: https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=ccpem > > Ludo > ----------- > ----------- > Hi all, > > > When we are talking about using the 3D classification to distinguish the > complex with/without ligand, could we introduce two different initial > model (with/without ligand) as references to relion 3D classification? > > > > > -----Original Message----- > From: Collaborative Computational Project in Electron cryo-Microscopy > [mailto:[log in to unmask]] On Behalf Of Molugu, Sudheer kumar > Sent: Friday, 21 November 2014 12:31 PM > To: [log in to unmask] > Subject: [ccpem] multi model refinements in RELION > > Hello, > > I was wondering if RELION can handle multimodel refinements. My data set > is heterogeneous and a mixture of three conformations. I am currently > using multirefine program in EMAN for my 3D model refinements. I would be > glad to use RELION if there is an inbuilt algorithm for refining more than > one model paralellely from the same raw data set. > > Thank you very much for your time. > > Best, > Sudheer Molugu Ph.D > cryoEM Facility Manager > Dept. of Chemistry > The University of Texas at El Paso > -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres