Dear All,

first i wish you nice weekend, then i wanna ask about formulation of my own mother liquor, i want to optimize a condition containing

 0.2m MgCl2, 0.1m Tris , PH 8; and 20% PEG6K as pricipitant. (PACT)

the question is , should i make a stock solution from every ingredient by dissolving it in miliQ water. then mix all stuff  together  and add water till final concentration with out adjusting final pH. OR dissolve the PEG in pH adjusted buffer and then mix all ingredients , so the final pH will be 8.

Amr