Hi Jeorge,
The simplest answer is that you have multiple positions for the Methionine.
So you can try adding in an alternate position for it. However, you didn’t
mention the sigma cutoff or e-/A^3 for either of your density maps. Its quite
possible that your fofc map is just scaled way down and what you are seeing is
an exaggeration of what’s barely there. Hope this helps. Cheers!
- Greg
-------------------------------------------------------------------------------
Greg
Costakes, Ph.D.
Department of Biochemistry
University of Cambridge
80
Tennis Court Road
Cambridge, CB2 1GA
United
Kingdom
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Sent: Wednesday, November 12, 2014 8:16 AM
Subject: [ccp4bb] Refinement with refmac05 and methionine
density
Dear All,
I am refining the structure with refmac05, and after each refinement the
density around this methionine is flipping. and it is difficult to say where
this methionine actually have the density. please suggest what I should I do. I
am attaching the snap here.
Thanks in Advance for your kind suggestions
Jeorge
