Since there is a hinge, you could try searching with the two domains
separately.
On 10/05/14 03:34, Luzuokun wrote:
> Dear all,
> I’m doing molecular replacement using Phaser. My protein is predicted
> to have two domain with a “hinge” linking them. The model sequence
> identity is 0.27. But the MR result is poor. I’ve tried other
> programme (Molrep, MrBump, Balbes,,,_.) But no improvement was
> observed. I think that this is due to the “open” or “closed”
> conformation around the hinge. I was told that I could place the Z
> axis along the hinge
> (http://xray0.princeton.edu/~phil/Facility/Guides/MolecularReplacement.html
> <http://xray0.princeton.edu/%7Ephil/Facility/Guides/MolecularReplacement.html>),
> could anyone tell me more details about how to do next?
>
> Thanks!
> Lu Zuokun
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All Things Serve the Beam
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David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
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