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Hi, Since my crystallization condition contains PEG, I am trying to use PEG as one of my cryo for testing. For example, one crystallization condition is 0.2M Na2PO4, 20%PEG3350. I want to make 0.2M Na2PO4 and 40% PEG3350 as cryoprotectant. When I added 200ul salt from 1M stock, and 800ul PEG from 50% stock, mixed them, seems they stayed as emulsion (confirmed by microscope), no matter how I pipette or vortex them. I tried to incubate it at 40degree, seems it got worse. I am now trying to re-make the cryo from solid reagent, not solution stock. Will this be helpful? Or is there any other trick? I will really appreciate! Best, Xiao