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Dear Mailbase, What are your current specimen requirements for ACTH? Most labs on Assay Finder appear to state EDTA, send to lab on ice ASAP and freeze immediately. However, there has been work published suggesting that ACTH is stable for a number of hours in EDTA (see attached) and there was a FOCUS poster a number of years ago by Heynes and Henson from QMC, Nottingham that showed preliminary data supporting that ACTH is stable for up to 24h at room temp. Are we being over cautious? I'd like to get a consensus view before adopting a more lenient approach. Best wishes, Nicola Nicola Pullan Principal Clinical Biochemist Pathology Department (Biochemistry) Royal United Hospital Bath NHS Trust Combe Park BATH BA1 3NG Tel: 01225 824711 Email: [log in to unmask] ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ******************************************************************************************************************** ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/