Hi Chengmin, What about reset your environment variables? From:Chengmin Li <[log in to unmask]> Date:2014-09-13 17:11 Subject:[ccpem] Ctffind3 ERROR To:"CCPEM"<[log in to unmask]> Cc: Dear All, There is an problem I have never met before. When I use relion to run ctffind3 , I can not get the CTF values. And the output information seems really strange:"word too long". What does that mean? The date I test in another PC runs very well! The details are as follows: Executing: `which relion_run_ctffind` --i "test.star" --o "test_ctf.star" --ctfWin -1 --CS 2.7 --HT 300 --AmpCnst 0.1 --XMAG 49296 --DStep 14 --Box 512 --ResMin 100 --ResMax 7 --dFMin 5000 --dFMax 50000 --FStep 500 --dAst 0 --ctffind3_exe /home/clp/softwares/ctf/ctffind3_mp.exe & Using CTFFINDs executable in: /home/clp/softwares/ctf/ctffind3_mp.exe to estimate CTF parameters for the following micrographs: * mrc/14_2.mrc * mrc/14_4.mrc * mrc/16_2.mrc * mrc/16_4.mrc * mrc/raw.mrc Estimating CTF parameters using Niko Grigorieff's CTFFIND3 ... 000/??? sec ~~(,_,"> [oo]Word too long. 0/ 0 sec ...........~~(,_,">Word too long. 0/ 0 sec .......................~~(,_,">Word too long. 0/ 0 sec ....................................~~(,_,">Word too long. How could I fix this problem? Thanks and have a good weekend!