Hi guys,

A quick question about pdb deposition! My protein has a common ligand 'heme' which mismatches with the ligand in pdb CCD (Chemical Component Dictionary). However i didn't find any differences in it. Is that because of the positions of double bonds or hydrogen atoms, since my model don't have hydrogen atoms. Actually i replace the heme with exact one from CCD and run refmac which give me a new pdb file. Unfortunately, CCD still didn’t recognize the heme.

Solutions?

Thank you!!

Bing Wang