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On 04/09/14 19:06, Lie Ma wrote: > Hello all, > > I am trying to make a model of an oligosaccharide which contains several sugar monomers. I obtained the monomers from coot library and put them into the electron density. But I don't know how to make covalent bond between these monomers. If you are using Coot, you can use Extensions -> Modelling -> Make Link. If you sugar monomers are reasonably placed Coot's Sphere Refine will determine the correct link types on the fly (it doesn't believe what's in the PDB file). > > I tried to use PRODRG to generate a model and it gave me one big ligand consisting of several monomers. Yes, that's not the way to do it... > However, what I want is to have a chain contains several monomers, and each monomers acts like a residue. Indeed. Paul.