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Dear All, Greetings and apology for an off topic query. may request you all to kindly share with me a conventional protocol, by which i can calculate a total Glutathione content (oxidized + reduced) in an *in-vitro* reaction set up. all i find over internet are kit-based methods, which turns out to be quite expensive for couple of reactions i would like to do. thanks in advance kind regards Avinash Kale