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Hi Andy, one possibility is to use "The Thalamus Atlas" described in these publications: http://www.ncbi.nlm.nih.gov/pubmed/?term=krauth%5Bauth%5D+AND+thalam*%5Btitle%2Fabstract%5D http://www.ncbi.nlm.nih.gov/pubmed/22700756 The authors may provide you with the ROI you are looking for. Best wishes, Giulio -- Giulio Pergola, PhD Assistant Professor Department of Basic Medical Science, Neuroscience and Sense Organs University of Bari, 'Aldo Moro' Piazza Giulio Cesare, 11 70121 Bari Italy Tel: +39 080 5478548 Fax: +39 080 5593204 Il Luned́ 14 Luglio 2014 4:41, Andy Yeung <[log in to unmask]> ha scritto: Dear all, If I wish to make a ROI at specified thalamic nuclei, what maps/tools can I use? WFU pickatlas or Julich Anatomy do not divide thalamus into its nuclei. Thanks for advice! Best, Andy