Thanks for the suggestion! But I coregistered the structural image to the template first, so they seem to have the same voxel size and centre point location when I checked them via "Display". To make the processing time shorter, I just tried to normalise one T2 image to the T2 template. Their orientation look quite similar in Check Reg, but the error still came up. Another thing I've tried is, I first used ImCalc to create a mean image of all my cases after I manual set them in the similar orientation and location. Then I tried to coregister and normalise individual images to the mean image. Still, I got the same error in normalise. I'm so confused about this situation.
> Date: Thu, 3 Jul 2014 19:45:17 +0100
> From:
[log in to unmask]> Subject: Re: [SPM] SPM error in normalise (estimate &write)
> To:
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> Some comments: The voxel size of your data can be different from the voxel size of the templates. As David states, make sure though that 1 mm in template space corresponds to 1 mm in world / "true" space, otherwise you have to scale your raw data via "Display".
>
> Your error message arises if you load several template images which don't fit well together. Maybe you've selected the rat template plus another file like the SPM T1 template?
>
> If your data does not correspond well to the template due to different orientations, you should get an error message like below:
>
> Coarse Affine Registration..
>
> * - SPM8: spm_affreg ----------------------------------------------
>
> There is not enough overlap in the images
> to obtain a solution.
>
> Please check that your header information is OK.
> The Check Reg utility will show you the initial
> alignment between the images, which must be
> within about 4cm and about 15 degrees in order
> for SPM to find the optimal solution.
>
> ----------------------------------------- ... - ....
>
> Failed 'Normalise: Estimate & Write'
> Error using spm_affreg>error_message (line 474)
> insufficient image overlap
> In file "C:\Progs\spm8\spm_affreg.m" (v4152), function "error_message" at line 474.
> In file "C:\Progs\spm8\spm_affreg.m" (v4152), function "spm_affreg" at line 177.
> In file "C:\Progs\spm8\spm_normalise.m" (v4621), function "spm_normalise" at line 172.
> In file "C:\Progs\spm8\config\spm_run_normalise_estwrite.m" (v4152), function "spm_run_normalise_estwrite" at line 22.
>
>
> Best,
>
> Helmut