Hi, I would be very grateful for some feedback regarding my GLM model for a 3 (group) x 2 (condition) Mixed Effect ANOVA. I have been following the instructions based on the 2-groups, 2-levels per subject (2-way Mixed Effect ANOVA) as described on the GLM section of the fslwiki (http://fsl.fmrib.ox.ac.uk/fsl/fslwiki/GLM#ANOVA:_2-groups.2C_2-levels_per_subject_.282-way_Mixed_Effect_ANOVA.29). I also used this post to clarify the 3 group approach: (https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind1311&L=FSL&P=R86271&1=FSL&9=A&J=on&d=No+Match%3BMatch%3BMatches&z=4). Currently the set-up is like this (only 2 subjects per group included): EV1(con) EV2 (conX1&2) EV3(conX1&3) EV4(conX2&3) EV5(s1) EV6(s2) EV7(s3) ... G1C1 1 1 1 0 1 0 0 G1C2 -1 -1 -1 0 1 0 0 G1C1 1 1 1 0 0 1 0 G1C2 -1 -1 -1 0 0 1 0 G2C1 1 -1 0 1 0 0 1 G2C2 -1 1 0 -1 0 0 1 G2C1 1 -1 0 1 0 0 0 G2C2 -1 1 0 -1 0 0 0 G3C1 1 0 1 -1 0 0 0 G3C2 -1 0 -1 1 0 0 0 G3C1 1 0 1 -1 0 0 0 G3C2 -1 0 -1 1 0 0 0 C1 1 0 0 0 C2 0 1 0 0 C3 0 0 1 0 C4 0 0 0 1 I have a feeling that this approach isn't quite right. In the example on the fslwiki group is ommited as an EV but 1 is assigned to group 1 and -1 to group 2 which doesn't really allow for a third group. Is there a way around this? Any comments feedback would be greatly appreciated. Thanks!