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Hi Anderson, I'm using TBSS. I used the same sample and the same number of variables to perform similar analyses and everything worked. Here is the matrix: /NumWaves 6 /NumPoints 69 /PPheights 3.900000e+01 2.400000e+01 2.000000e+00 7.000000e+02 1.000000e+00 1.000000e+00 /Matrix 3.710000e+00 2.450000e+00 1.000000e+00 -1.389300e+02 1.000000e+00 0.000000e+00 -1.829000e+01 -7.550000e+00 1.000000e+00 2.610700e+02 1.000000e+00 0.000000e+00 7.710000e+00 -4.550000e+00 -1.000000e+00 6.107000e+01 1.000000e+00 0.000000e+00 7.100000e-01 6.450000e+00 -1.000000e+00 -1.389300e+02 1.000000e+00 0.000000e+00 -7.290000e+00 -7.550000e+00 -1.000000e+00 -8.893000e+01 1.000000e+00 0.000000e+00 3.710000e+00 5.450000e+00 -1.000000e+00 -2.139300e+02 1.000000e+00 0.000000e+00 -6.290000e+00 5.450000e+00 1.000000e+00 -1.639300e+02 1.000000e+00 0.000000e+00 2.710000e+00 -3.550000e+00 -1.000000e+00 6.107000e+01 1.000000e+00 0.000000e+00 -6.290000e+00 4.450000e+00 -1.000000e+00 6.107000e+01 1.000000e+00 0.000000e+00 6.710000e+00 4.500000e-01 1.000000e+00 -8.893000e+01 1.000000e+00 0.000000e+00 -5.290000e+00 -2.550000e+00 1.000000e+00 -1.389300e+02 1.000000e+00 0.000000e+00 6.710000e+00 6.450000e+00 1.000000e+00 2.110700e+02 1.000000e+00 0.000000e+00 -3.290000e+00 -2.550000e+00 1.000000e+00 1.107000e+01 1.000000e+00 0.000000e+00 -5.290000e+00 -6.550000e+00 1.000000e+00 4.610700e+02 1.000000e+00 0.000000e+00 1.571000e+01 -7.550000e+00 -1.000000e+00 1.610700e+02 1.000000e+00 0.000000e+00 2.710000e+00 3.450000e+00 -1.000000e+00 -3.893000e+01 1.000000e+00 0.000000e+00 2.071000e+01 1.045000e+01 -1.000000e+00 -1.514300e+02 1.000000e+00 0.000000e+00 1.710000e+00 8.450000e+00 1.000000e+00 -1.889300e+02 1.000000e+00 0.000000e+00 -1.529000e+01 -2.550000e+00 1.000000e+00 -1.889300e+02 1.000000e+00 0.000000e+00 2.071000e+01 -4.550000e+00 -1.000000e+00 2.610700e+02 1.000000e+00 0.000000e+00 2.710000e+00 1.245000e+01 1.000000e+00 -1.889300e+02 1.000000e+00 0.000000e+00 4.710000e+00 5.450000e+00 1.000000e+00 -3.893000e+01 1.000000e+00 0.000000e+00 -2.900000e-01 -4.550000e+00 -1.000000e+00 1.610700e+02 1.000000e+00 0.000000e+00 3.710000e+00 -5.550000e+00 1.000000e+00 1.610700e+02 1.000000e+00 0.000000e+00 1.571000e+01 1.445000e+01 1.000000e+00 2.610700e+02 1.000000e+00 0.000000e+00 -2.900000e-01 1.450000e+00 1.000000e+00 -1.889300e+02 1.000000e+00 0.000000e+00 7.100000e-01 9.450000e+00 1.000000e+00 -1.389300e+02 1.000000e+00 0.000000e+00 1.371000e+01 -1.550000e+00 -1.000000e+00 -5.930000e+00 1.000000e+00 0.000000e+00 -1.029000e+01 -5.550000e+00 1.000000e+00 -1.389300e+02 1.000000e+00 0.000000e+00 -5.290000e+00 1.450000e+00 1.000000e+00 -1.389300e+02 1.000000e+00 0.000000e+00 1.771000e+01 7.450000e+00 -1.000000e+00 -8.893000e+01 1.000000e+00 0.000000e+00 4.710000e+00 4.500000e-01 1.000000e+00 -1.389300e+02 1.000000e+00 0.000000e+00 -7.290000e+00 -6.550000e+00 1.000000e+00 -3.893000e+01 1.000000e+00 0.000000e+00 -1.129000e+01 -9.550000e+00 1.000000e+00 -3.893000e+01 1.000000e+00 0.000000e+00 1.371000e+01 4.500000e-01 1.000000e+00 4.610700e+02 1.000000e+00 0.000000e+00 -9.290000e+00 -4.550000e+00 1.000000e+00 2.610700e+02 1.000000e+00 0.000000e+00 -5.290000e+00 -5.500000e-01 -1.000000e+00 -1.389300e+02 1.000000e+00 0.000000e+00 7.100000e-01 -2.550000e+00 1.000000e+00 3.610700e+02 1.000000e+00 0.000000e+00 2.710000e+00 9.450000e+00 -1.000000e+00 2.610700e+02 1.000000e+00 0.000000e+00 5.710000e+00 1.450000e+00 1.000000e+00 4.110700e+02 1.000000e+00 0.000000e+00 -9.290000e+00 -2.550000e+00 1.000000e+00 -1.389300e+02 1.000000e+00 0.000000e+00 2.710000e+00 -5.500000e-01 1.000000e+00 -3.893000e+01 1.000000e+00 0.000000e+00 1.471000e+01 -1.550000e+00 -1.000000e+00 6.107000e+01 1.000000e+00 0.000000e+00 -1.429000e+01 -9.550000e+00 1.000000e+00 -1.389300e+02 1.000000e+00 0.000000e+00 -2.900000e-01 -8.550000e+00 -1.000000e+00 -1.889300e+02 1.000000e+00 0.000000e+00 -7.290000e+00 3.450000e+00 1.000000e+00 6.107000e+01 1.000000e+00 0.000000e+00 -1.290000e+00 -3.550000e+00 1.000000e+00 -3.893000e+01 1.000000e+00 0.000000e+00 -1.529000e+01 -4.550000e+00 -1.000000e+00 -1.889300e+02 1.000000e+00 0.000000e+00 -2.900000e-01 9.450000e+00 -1.000000e+00 -3.893000e+01 0.000000e+00 1.000000e+00 -3.290000e+00 5.450000e+00 1.000000e+00 -2.389300e+02 0.000000e+00 1.000000e+00 4.710000e+00 -5.500000e-01 1.000000e+00 2.610700e+02 0.000000e+00 1.000000e+00 -8.290000e+00 -5.500000e-01 1.000000e+00 3.610700e+02 0.000000e+00 1.000000e+00 7.100000e-01 7.450000e+00 -1.000000e+00 6.107000e+01 0.000000e+00 1.000000e+00 -2.900000e-01 -7.550000e+00 1.000000e+00 -1.389300e+02 0.000000e+00 1.000000e+00 -2.290000e+00 -5.550000e+00 1.000000e+00 -8.893000e+01 0.000000e+00 1.000000e+00 1.271000e+01 -8.550000e+00 1.000000e+00 -3.893000e+01 0.000000e+00 1.000000e+00 -1.329000e+01 -5.500000e-01 1.000000e+00 -1.889300e+02 0.000000e+00 1.000000e+00 8.710000e+00 2.450000e+00 1.000000e+00 -3.893000e+01 0.000000e+00 1.000000e+00 -7.290000e+00 -8.550000e+00 1.000000e+00 -8.893000e+01 0.000000e+00 1.000000e+00 5.710000e+00 4.500000e-01 1.000000e+00 1.610700e+02 0.000000e+00 1.000000e+00 -5.290000e+00 1.450000e+00 -1.000000e+00 -1.889300e+02 0.000000e+00 1.000000e+00 -2.900000e-01 -4.550000e+00 1.000000e+00 2.610700e+02 0.000000e+00 1.000000e+00 -8.290000e+00 3.450000e+00 -1.000000e+00 -2.059300e+02 0.000000e+00 1.000000e+00 1.710000e+00 4.500000e-01 1.000000e+00 -1.889300e+02 0.000000e+00 1.000000e+00 -1.829000e+01 -7.550000e+00 -1.000000e+00 -1.389300e+02 0.000000e+00 1.000000e+00 -1.029000e+01 1.450000e+00 1.000000e+00 1.110700e+02 0.000000e+00 1.000000e+00 -2.290000e+00 -1.550000e+00 1.000000e+00 -1.389300e+02 0.000000e+00 1.000000e+00 -2.900000e-01 1.450000e+00 1.000000e+00 2.610700e+02 0.000000e+00 1.000000e+00 1.271000e+01 1.245000e+01 1.000000e+00 -2.059300e+02 0.000000e+00 1.000000e+00 Thanks! 2014-07-15 17:13 GMT+02:00 Anderson M. Winkler <[log in to unmask]>: > Hi Irene, > Just to be sure, could you please post your design matrix here and tell > whether this study is using VBM, TBSS, fMRI or yet something else? Thanks! > Anderson > > > > On 15 July 2014 15:09, Irene Bollettini <[log in to unmask]> > wrote: > >> Hi Anderson, >> thanks for your response. >> I waited for one week, just for the contrast 3, and the analysis didn't >> run out. >> It surprises me, because I performed analyses with much more subjects >> (till 130 subjects) using the same workstation and this message didn't >> appear. >> >> Thank you again, >> best >> >> Irene >> >> >> 2014-07-15 15:38 GMT+02:00 Anderson M. Winkler <[log in to unmask]>: >> >> Hi Irene, >>> This is just a warning, indicating that it will take longer for these >>> contrasts to run. It's not an error, and if you see the results at the end, >>> it's fine. >>> All the best, >>> Anderson >>> >>> >>> >>> On 15 July 2014 12:21, Irene Bollettini <[log in to unmask]> >>> wrote: >>> >>>> Dear FSL experts, >>>> >>>> while running randomise, this message appears: >>>> >>>> "2.69808e+17 permutations required for exhaustive test of t-test 1 >>>> Doing 5000 random permutations >>>> Starting permutation 1 (Unpermuted data) >>>> Starting permutation 2 >>>> Warning: tfce has detected a large number of integral steps. This >>>> operation may require a great deal of time to complete" >>>> >>>> This message appears for the contrast 3, 4, 5 and 6, whereas the first >>>> two contrasts work. >>>> >>>> The order of the images matches the design.con and design.mat files. >>>> I attached the commands below >>>> >>>> *Design.mat. * >>>> NumWaves 6 >>>> /NumPoints 69 >>>> /PPheights 3.900000e+01 2.400000e+01 2.000000e+00 7.000000e+02 >>>> 1.000000e+00 1.000000e+00 >>>> >>>> >>>> >>>> *Design.con* >>>> >>>> /ContrastName1 m>v >>>> /ContrastName2 m<v >>>> /ContrastName3 +m >>>> /ContrastName4 -m >>>> /ContrastName5 +v >>>> /ContrastName6 -v >>>> /NumWaves 6 >>>> /NumContrasts 6 >>>> /PPheights 1.207759e+00 1.207759e+00 1.634991e+00 >>>> 1.634991e+00 1.367166e+00 1.367166e+00 >>>> /RequiredEffect 1.267 1.267 0.960 0.960 1.238 >>>> 1.238 >>>> >>>> *Matrix* >>>> >>>> 0.000000e+00 0.000000e+00 0.000000e+00 0.000000e+00 1.000000e+00 >>>> -1.000000e+00 >>>> 0.000000e+00 0.000000e+00 0.000000e+00 0.000000e+00 -1.000000e+00 >>>> 1.000000e+00 >>>> 0.000000e+00 0.000000e+00 0.000000e+00 0.000000e+00 1.000000e+00 >>>> 0.000000e+00 >>>> 0.000000e+00 0.000000e+00 0.000000e+00 0.000000e+00 -1.000000e+00 >>>> 0.000000e+00 >>>> 0.000000e+00 0.000000e+00 0.000000e+00 0.000000e+00 0.000000e+00 >>>> 1.000000e+00 >>>> 0.000000e+00 0.000000e+00 0.000000e+00 0.000000e+00 0.000000e+00 >>>> -1.000000e+00 >>>> >>>> >>>> Could you help me? >>>> >>>> thank you, >>>> Irene >>>> >>>> -- >>>> Irene Bollettini >>>> PhD student >>>> Scientific Institute and University Vita-Salute San Raffaele >>>> Deptartment of Clinical Neurosciences >>>> San Raffaele Turro >>>> via Stamira d'Ancona 20 >>>> 20127 Milano Italy >>>> tel +39 02 26433156 >>>> fax +39 02 26433265 >>>> E-mail *[log in to unmask] <[log in to unmask]>* >>>> >>> >>> >> >> >> -- >> Irene Bollettini >> PhD student >> Scientific Institute and University Vita-Salute San Raffaele >> Deptartment of Clinical Neurosciences >> San Raffaele Turro >> via Stamira d'Ancona 20 >> 20127 Milano Italy >> tel +39 02 26433156 >> fax +39 02 26433265 >> E-mail *[log in to unmask] <[log in to unmask]>* >> > > -- Irene Bollettini PhD student Scientific Institute and University Vita-Salute San Raffaele Deptartment of Clinical Neurosciences San Raffaele Turro via Stamira d'Ancona 20 20127 Milano Italy tel +39 02 26433156 fax +39 02 26433265 E-mail *[log in to unmask] <[log in to unmask]>*