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Dear Gajana, you can use . PISA ((Krissinel and Henrick, 2007) (EMBL server) and NACCESS (Hubbard and Thornton, 1993). (not for windows i think). Hope to help Nicolas ________________________________________ De : CCP4 bulletin board [[log in to unmask]] de la part de Gajanan Arbade [[log in to unmask]] Envoyé : mardi 1 juillet 2014 20:49 À : [log in to unmask] Objet : [ccp4bb] Buried surface area calculation.. Hello, Am working with some DNA binding proteins. If I want to calculate the buried surface area, which software tools should i use? Suggest me some windows based programs to solve my purpose. Thank you & Regards, Gajanan __________________________________________ Gajanan K Arbade Research Scholar Defence Institute of Advanced Technology (DIAT) Pune Maharashtra, India Pin Code-411025 Contact No. Lab.+ 91-20-24304377 Mob: 08698198016