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You may need to 1) modify _chem_comp.group to be "DNA" in the cif-file 2) import the resulting cif-file in coot Cheers Sent on a Sprint Samsung Galaxy S® III <div>-------- Original message --------</div><div>From: "Wang, Wei" <[log in to unmask]> </div><div>Date:07/15/2014 5:14 AM (GMT-05:00) </div><div>To: [log in to unmask] </div><div>Subject: [ccp4bb] The modified DNA could not be linked to the downstream DNA in the COOT? </div><div> </div>Hi, There is a problem about modified DNA refinement. I generated one CIF file of a modified DNA base using eLBOW software of PHENIX. However I found the modified DNA could not be linked to the downstream DNA when I refined it in the COOT. Then I generated another CIF file using sketcher of CCP4, but the problem still existed. Is there any expert to help me? Thanks!! Best Wei