Dear All,<br />I have been trying to answer the following questions:<br />1) In the context of optical imaging, how is a protein crystallography reconstruction validated? How meaningful is this perspective of protein crystallography (optical imaging)?<br />2) In transition to single particles (single unit cells), what additional factors should be considered?<br />I am not a chemist or a crystallographer, and the question for me is the applicability/accuracy of the scattering model(s). I will be grateful if you could comment on my below-mentioned answers to these questions. For a quick check, I would suggest &quot;Abstract + Introduction + Tables 1 &amp; 2 + Conclusions&quot;.<br /><br />Thank you very much for your time in advance.<br /><br /><br />The legacy of protein crystallography<br />http://arxiv.org/ftp/arxiv/papers/1407/1407.6595.pdf<br /><br />Beyond protein crystallography<br />http://arxiv.org/ftp/arxiv/papers/1407/1407.6596.pdf<br />