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Hi Ludo, Have you checked the mask you're using? Also, what are the B-factor in the bfactors.star file? S > Hi, > I'm trying to process a cleaned up 250K particles dataset with the new > movie processing approach before doing 3d classification. > Data was collected with titan krios, epu, falcon2, 7 frames, 8 electron > dose per frame. > I followed the steps described in the movie processing to extract movie > frames and resume a 3d refinement using movie frames. > I wanted to use frames 2-5 only (whole is 1-7) and then put a running > average of 1. This should be fine? > Then I did the polishing step. again running avg of 1. > I can see all the files created, even the shiny.star, but I get a > > + randomize phases beyond: -286.72 Angstroms > Postprocessing::run ERROR: FSC curve never drops below randomize_fsc_at > File: src/postprocessing.cpp line: 651 > > Since I have the unfiltered half maps created, I wanted to look at them, > but chimera can't open them: LinAlgError:Singular matrix. > This error happens with each unfilt maps created at this step. > > What would be the best way to try identfying the problem? > Thanks, > Ludo > -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres