Dear ccp4 users,

I get the following message from Phaser when I do a molecular replacement with two ensembles. One of the ensembles is placed but the second one is not placed, and then it says this:

Solutions with Z-scores greater than 13.0 (the threshold indicating a definite

solution) were rejected for failing packing test

#1 TFZ=16.8 PAK=487

#2 TFZ=17.4 PAK=440

#3 TFZ=17.0 PAK=312

#4 TFZ=16.7 PAK=294

#5 TFZ=16.8 PAK=227

#6 TFZ=16.6 PAK=284

#7 TFZ=16.2 PAK=219

#8 TFZ=16.3 PAK=287

#9 TFZ=16.1 PAK=186

#10 TFZ=16.6 PAK=277

#11 TFZ=16.7 PAK=204

#12 TFZ=16.8 PAK=404

#13 TFZ=15.9 PAK=271

#14 TFZ=15.8 PAK=194

#15 TFZ=14.9 PAK=229

#16 TFZ=16.4 PAK=368

#17 TFZ=17.3 PAK=194

#18 TFZ=15.2 PAK=240

#19 TFZ=16.1 PAK=325

#20 TFZ=15.3 PAK=455

#21 TFZ=16.5 PAK=298

#22 TFZ=16.6 PAK=290

#23 TFZ=15.2 PAK=259

#24 TFZ=16.2 PAK=194

#25 TFZ=16.1 PAK=314

#26 TFZ=16.3 PAK=194

#27 TFZ=16.3 PAK=387

#28 TFZ=15.6 PAK=193

#29 TFZ=15.7 PAK=219

#30 TFZ=15.6 PAK=474

#31 TFZ=15.1 PAK=194

#32 TFZ=15.4 PAK=339

#33 TFZ=15.5 PAK=210

#34 TFZ=15.2 PAK=300

#35 TFZ=15.6 PAK=186

#36 TFZ=16.2 PAK=216

#37 TFZ=14.9 PAK=182

#38 TFZ=15.7 PAK=279

#39 TFZ=15.5 PAK=285

#40 TFZ=15.0 PAK=374

#41 TFZ=15.4 PAK=404

#42 TFZ=15.3 PAK=185

#43 TFZ=15.6 PAK=227

#44 TFZ=14.8 PAK=364

#45 TFZ=15.7 PAK=193

#46 TFZ=14.5 PAK=448

#47 TFZ=14.6 PAK=219

#48 TFZ=15.5 PAK=210

#49 TFZ=15.7 PAK=189

#50 TFZ=15.0 PAK=193

#51 TFZ=15.0 PAK=281

#52 TFZ=15.2 PAK=202


And the list still continues for a bit. How should I think about this? I would assume that the clashes are too many only by seeing those numbers, but maybe there is something else to take into account?

Thanks a lot in advance.

Best wishes,

Almudena



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Almudena Ponce-Salvatierra
Macromolecular crystallography and Nucleic acid chemistry
Max Planck Institute for Biophysical Chemistry
Am Fassberg 11 37077 Göttingen
Germany