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Dear Huang, I really didn't understand about this parameter ..128*128*7*600. You do the slice timing correction ,,,this way. Session> put your fMRI images...(I guess you might be having 128 volumes) No. of slices...>>enter ur value; say for eg. 24 TR>> enter ur TR; eg. 4 sec TA>> use the formula TR-(TR/nslices). SO here it will be 4-(4/24) Slice order>> Check in ur fMRI parameter, whether u 're using ascending, descending or interleaved seq. then use the formula accordingly. From ur eg. I think u're using interleaved ;bottom to top seq. Reference slice>> 1 (u can use ur first slice as ref. ) On Thu, May 29, 2014 at 10:23 PM, shiliang huang <[log in to unmask]> wrote: > Hi Expert > I want to use SPM8 to do slice timing. My dataset is 128*128*7*600. The > slice order when I acquired my data is [1 3 5 7 2 4 6]. should I set the > Slice order as [1 3 5 7 2 4 6] when I use SPM8? Which slice is used as the > 'Reference Slice'? > Thanks, > Qiang >