Dear FSL experts, I desperately need your help! I am trying to preform group ICA on resting state data using Melodic and have been running into a serious difficulty. While melodic seems to work fine for single subject analysis or even group ICA on only a limited number of participants it is unable to run my entire data set (n=73). At the very start of the analysis the progress report appears to freeze at: /bin/cp /tmp/feat_5cCO9X.fsf design.fsf mkdir .files;cp /Applications/fsl/doc/fsl.css .files;ln -s /Applications/fsl/doc/images .files/images Melodic progress report for the first subject generates this error message: /Applications/fsl/bin/fslmaths C/subj_011/SE9/rest prefiltered_func_data -odt float Cannot open volume C/subj_011/SE9/rest for reading! FATAL ERROR ENCOUNTERED Cannot open volume C/subj_011/SE9/rest for reading! while executing "if { [ catch { for { set argindex 1 } { $argindex < $argc } { incr argindex 1 } { switch -- [ lindex $argv $argindex ] { -I { incr argin..." (file "/Applications/fsl/bin/feat" line 119) I have no idea what it means by ³cannot open volume for reading² because, as mentioned previously, the dataset (including subj_011) works fine for many other types of analysis. If anyone else has had a similar problem or may suspect what the matter is please let me know. Thanks in advance and a happy Easter, John -------------------- John Broulidakis Developmental Brain-Behaviour Laboratory University of Southampton