I have a high resolution (2A) native structure of a protein and structure factors for a relatively low resolution (2.6A) of the same protein bound with its substrate (complex) having same space group and cell parameters (P212121 is the space group and cell parameters are a,b,c =39.4,64.5,117.2. and a,b,c = 39.6,63.9,117.8 for native and complex)..My question is ..what is the best way to solve the complex structure ??..whether it should be done through molecular replacement with the native structure or simply by doing rigid body refinement through mtz from complex and coordinates of the native structures..in summary...what should be the ideal method employed for the structure solution if the aim is to compare between the native and complex structure of the same protein ??.

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Regards

Faisal
School of Life Sciences
JNU