Cheers,
_______________________________________
Roger S. Rowlett
Gordon & Dorothy Kline Professor
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: [log in to unmask]
[log in to unmask]" type="cite">Dear All,
I want to get some help regarding crystallization for one of the protein I am working. This is a recombinant protein of molecular weight of 17.5 kDa. I am getting crystals shape like thin plates in 5 days, in different conditions like bis tris pH 6.5-8, HEPES pH 6-8 with .1M nacl , .01M DTT having PEG 3350, PEG 6000 as precipitant. But whenever we used to put crystal in cryoprotectant like 20 % ethylene glycol, glycerol, MPD it used to split like layers of some tree barks.And the crystal which were diffracting not getting diffracted above 3.0 Angstrom.
--Please help me out.
Thanks in advance
Anamika