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Hi, I am wondering if it is known what is the slowest step in the calculations by relion. Is there a sub-routine/function which is the bottleneck for the 2D class averages/3d class averages and 3d auto refinement? Has anyone compiled and run relion with gprof to identify the slowest step? I am asking because if one would want to re-write part of the code for the GPUs, one needs to know which step will benefit the most from such an optimization. Thanks, Klim