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Hi Ming, The default of post process is only to filter to the average resolution. That will make things indeed "scattered" in areas that are more flexible than the average. You can however filter the map to any resolution (and factor) you want using the --lowpass and --adhoc_bfac options. Local resolution filtering is not implemented. HTH, S > Hi All > > I recently have problems in relion post processing. The reported > resolution > and mask are reasonable, but the output mrc does not look that good. The > peripheral regions are very scattered. > Could it be possible that these parts are not filtered properly, saying > filtered to average resolution, but not local resolution? > Also I'm thinking of doing local b-factor sharpening and filter. Could > RELION do it? > (Maybe they have been implemented. just I did not find it....) > > Thanks again! > > -Ming > > > -- > > Ming Sun > Ph.D Candidate, Joachim Frank Group > Department of Biochemistry and Molecular Biophysics, > and Department of Biological Sciences, Columbia University > (E): [log in to unmask] > (T): 718-974-3686 > -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres