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Hello Shanti, Typically, one can safely claim a solved structure once an interpretable electron density map is obtained to which most of your protein model can be fitted properly. The primary numeric indication for that are your R-factors. The high values you have suggest that either something is wrong with your space group, twinning, etc. as Eleanor suggested, or your space group and MR solution may be still correct but for a search model of poor similarity with your crystal structure. Even in the later case, at this resolution it is going to be very challenging to build and refine a structure to reasonable R-factors starting from a poor model. Hay ======================================== Hay Dvir Ph. D. Head Technion Center for Structural Biology Technion Haifa 3200003, Israel Tel: +(972)-77-887-1901 Fax: +(972)-77-887-1935 E-mail [log in to unmask] Website http://tcsb.technion.ac.il/Hay-Dvir ======================================== On Feb 4, 2014, at 11:36 AM, Shanti Pal Gangwar wrote: > Dear All > > I have solved one structure by MR. The data data quality was poor so the Rmerge was high. The resolution of the data is 3.3 Angstrom.The refinement statistics are also very poor Rw/Rf= 40/42 %. After many efforts we are not able to get reasonable Rw/Rf. > > My question is "can it be claimed that we have solved the structure?" > > > Thanks in advance > > > > > > -- > ******************** > regards > Shanti Pal Gangwar > School of Life Sciences > Jawaharlal Nehru University > New Delhi-110067 > India > Email:[log in to unmask] > >