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Dear Joern and other BBers, While I fully agree that it is important to test a few images at room temperature, to know the crystal's potential, I think that almost always it will be possible to achieve better diffraction using cryogenic data collection. Those rare cases, as the one you mention below are worthy of critical investigation as to why there is a loss of order on cryo-cooling: Unsuitable cryoprotectant is my first guess. The rate of cooling in liquid N2 is slow, liquid ethane could be a better choice. Enrico On Thu, 06 Feb 2014 11:19:47 +0100, Joern Krausze <[log in to unmask]> wrote: > Dear Theresa, > > We recently collected a room temperature data set from one single > crystal at Petra III. The beam line was equipped with a Pilatus > detector. Data were good to 2.7 A. In contrast, at 100 K similar > crystals diffracted very poorly. So, it is perfectly possible to obtain > useful room temperature data sets from synchrotron sources. I have to > admit that in our case it certainly helped that the crystal belonged to > a high-symmetry space and full completeness was achieved after 40 > degrees angular range. > > Regards, > > Joern > > Sent from my iPad > >> On 06.02.2014, at 10:51, Theresa Hsu <[log in to unmask]> wrote: >> >> Dear crystallographers >> >> Just out of curiosity, is it possible to collect datasets from crystals >> at room temperature at synchrotron? Are fast detectors like Pilatus >> useful for this? >> >> Thank you. >> >> Theresa -- Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab http://www-dsv.cea.fr/ibitecs/simopro/ltmb/cristallogenese LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE http://scholar.google.com/citations?hl=en&user=Kvm06WIoPAsC&pagesize=100&sortby=pubdate http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html e-mail: [log in to unmask] Fax: 33 (0)1 69 08 90 71