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*********************** This message has been scanned by the InterScan for CSC SSM by IICT security policy and found to be free of known security risks. *********************** Dear Annemarie, This could be a reaction product of the PMSF with either tris or alanine (as you have modeled, but you should know the source). Anthony ----------------------------------------------------- Dr. Anthony Addlagatta Center for Chemical Biology Indian Institute of Chemical Technology [IICT] Tarnaka, Hyderabad AP-500 607, INDIA Tel:91-40-27191812 Web: https://sites.google.com/site/chembioliict/home/dr-anthony-addlagatta-1 ---------- Original Message ----------- From: Annemarie Weber <[log in to unmask]> To: [log in to unmask] Sent: Mon, 3 Feb 2014 17:37:00 +0100 Subject: [ccp4bb] unexplained density > *********************** > This message has been scanned by the InterScan for CSC SSM at IICT and found to be free of known security risks. > *********************** > > Dear all, > I am refining a 1.4 A resolution structure and found some well-defined > but unfortunately unexplained density. The protein was purified in HEPES > buffer with PMSF and protease inhibitor cocktail tablets (Roche) added. > It was cocrystallized with AMPPNP in PEG2000MME and Tris. I modelled a > molecule into the density, which fits quite well but I do not know what > it is and how it got there. Attached are two pictures with the > difference density and the molecule I modelled in there. > Has anybody seen something like this before? Maybe some degradation > product of the buffer/PMSF? > Any suggestions will be highly welcome. > Thanks a lot > Annemarie ------- End of Original Message ------- ------------------------------------------------------------------------------------------------- Note: The information contained in the e-Mail message and/or attachments to it may contain confidential or privileged information. If you are not the intended recipient, any dissemination, use, review, distribute, prinitng or copying of the information contianed in this e-Mail message and/or attachments to it are strictly prohibited. If you have received this communication in error. Please notify us by reply e-Mail or telephone and immediately and permanently delete the message and any attachment. Thank you.