Dear Donald and Chao-gan£¬ Very grateful for your help! Following your instructions, the out-of-shape normalized images have been greatly improved, and problem has plagued me solved. Thank you very much£¡ Lei. At 2013-12-06 04:01:38,"MCLAREN, Donald" <[log in to unmask]> wrote: You need to make sure the the origin is at the AC and the rotation matches the MNI template. If you are using the T1 segmentation parameters for normalization, then you need to make sure that the T1 and fMRI data are co-registered. Best Regards, Donald McLaren ================= D.G. McLaren, Ph.D. Research Fellow, Department of Neurology, Massachusetts General Hospital and Harvard Medical School Postdoctoral Research Fellow, GRECC, Bedford VA Website: http://www.martinos.org/~mclaren Office: (773) 406-2464 ===================== This e-mail contains CONFIDENTIAL INFORMATION which may contain PROTECTED HEALTHCARE INFORMATION and may also be LEGALLY PRIVILEGED and which is intended only for the use of the individual or entity named above. If the reader of the e-mail is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you are in possession of confidential and privileged information. Any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited and may be unlawful. If you have received this e-mail unintentionally, please immediately notify the sender via telephone at (773) 406-2464 or email. On Thu, Dec 5, 2013 at 2:54 PM, Lei Gao <[log in to unmask]> wrote: Dear SPM experts, I encountered a very tricky problem, I processed the fMRI data with SPM8, including format convert, slice timing, head motion correction spatial normalization to the Montreal Neurological Institute (MNI) template (resampling voxel size = 3¡Á3¡Á3 mm3), unfortunately, I found the functional volumes can't be normalized (both EPI template and T1 segement). I want to know where may be wrong and the possible solutions. thanks in advance! Lei.