Hi Sjors, the images in our dataset are not well centered. This might create problems when using a tight mask in 2D classification runs (part of the particles are masked out. Is there an (easy) way for correcting this? Thanks, Dieter - ------------------------------------------------------------------------ Dieter Blaas, Max F. Perutz Laboratories Medical University of Vienna, Inst. Med. Biochem., Vienna Biocenter (VBC), Dr. Bohr Gasse 9/3, A-1030 Vienna, Austria, Tel: 0043 1 4277 61630, Fax: 0043 1 4277 9616, e-mail: [log in to unmask] ------------------------------------------------------------------------