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Hi Nazia,

you did not say what Rwork and Rfree values are... If, for instance, Rwork=20% and Rfree=21% then a situation when Rfree stays the same or even increases and Rwork goes down is totally valid as long as a reasonable Rfree-Rwork gap is maintained. 
The point is that you need to look at three numbers Rwork, Rfree and Rfree-Rwork, not either one of them.

Pavel


On Mon, Dec 9, 2013 at 10:51 PM, Nazia Nasir Phd2009,ProteinCrystall.Lab <[log in to unmask]> wrote:
Hi,

I am facing a problem in refining one of my structures. With every
refinement the Rwork is going down. However, the Rfree is stuck and is
not going down with refinement. I have already solved the structure
without the co-factor bound. This structure is with the co-factor
bound. Can anyone suggest the reason for the Rfree getting stuck?

Thanks

--
Nazia Nasir
PhD Scholar
Protein Crystallography Lab
National Institute of Immunology
New Delhi