----------------------------------------- Dr. Matthias Zebisch Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK Phone (+44) 1865 287549; Fax (+44) 1865 287547 Email [log in to unmask] Website http://www.strubi.ox.ac.uk -----------------------------------------On 12/10/2013 12:44 PM, PriyankMaindola wrote:
[log in to unmask]" type="cite">dear members
i am trying to solve this crystal structure butI am puzzled with anundefined blob thatappeared at a glutamine residue after refinement. I have attached pics of that below.Is it a covalent modification of acid-amide side chain..... as there is no charged environment around and density seems continuous.
please suggest....
following reagents were encountered by protein during purification, crystallization and soaking:
phenyl methyl sulfonyl fluoride
benzamidine
tris
dtt (could it be cyclized dtt?)
k[au(cn)2]
acidic pH
isopropanol
citrate
sulfate
phosfate
K+, Na+, Cl-
map contour:
2fo-fc: 1rmsd
fo-fc (green): 3 rmsd
--Priyank