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​

dear members

i am trying to solve this crystal structure but 

​I am puzzled with an​

 undefined  blob

​ that​

 

​appeared at a glutamine residue after refinement. I have attached pics of that below. 

Is it a covalent modification of acid-amide side chain..

​... as there is no charged environment around and density seems continuous.

​please suggest

​

....

following reagents were encountered by protein

​ during purification, crystallization and soaking​

:

phenyl methyl sulfonyl fluoride

benzamidine

tris

dtt

​  (could it be cyclized dtt?)​

k[au(cn)2]

acidic pH

isopropanol

citrate

sulfate

phosfate

K+, Na+, Cl-

​ map contour:
2fo-fc: 1rmsd

fo-fc (green): 3 rmsd​

-- 

Priyank

-- 
Fred. Vellieux (B.Sc., Ph.D., hdr)

IBS2 / ELMA
Campus EPN
6 rue Jules Horowitz
F-38042 Grenoble
Tel: +33 457428605
(Fax: +33 438785494)