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Hi Nazia, you did not say what Rwork and Rfree values are... If, for instance, Rwork=20% and Rfree=21% then a situation when Rfree stays the same or even increases and Rwork goes down is totally valid as long as a reasonable Rfree-Rwork gap is maintained. The point is that you need to look at three numbers Rwork, Rfree and Rfree-Rwork, not either one of them. Pavel On Mon, Dec 9, 2013 at 10:51 PM, Nazia Nasir Phd2009,ProteinCrystall.Lab < [log in to unmask]> wrote: > Hi, > > I am facing a problem in refining one of my structures. With every > refinement the Rwork is going down. However, the Rfree is stuck and is > not going down with refinement. I have already solved the structure > without the co-factor bound. This structure is with the co-factor > bound. Can anyone suggest the reason for the Rfree getting stuck? > > Thanks > > -- > Nazia Nasir > PhD Scholar > Protein Crystallography Lab > National Institute of Immunology > New Delhi >