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> I am currently using the longitudinal registration tool from SPM 12 in > order to estimate GM volumes changes between 2 time points and relate these > changes to clinical measures. > > Once I created the Jacobian difference image, I understood I should > multiply the volume differences image by the GM volumes but I can't get > how. > > 1/ Which GM volume shall I choose (Time 1 or the mid point average) ? > To avoid bias, the longitudinal registration aligns the scans to the mid-point average. All the Jacobians etc are in this average space. Therefore, I'd suggest that for any segmenting, you should use the mid-point average. 2/ Shall I use the total GM volume or voxel by voxel ? > The aim is to identify regional grey matter volume changes, so you'd multiply the GM maps by the volume change maps (Jacobian differences) to give maps of GM volume change. 3/ Which tool shall I use to do this in SPM ? > If you don't have so many subjects, you could multiply the images together via ImCalc (evaluating i1.*i2). If there are many subjects, you may want to try some form of MATLAB scripting. Best regards, -John